Beilstein J. Nanotechnol.2015,6, 383–395, doi:10.3762/bjnano.6.38
functionalized polystyrene nanoparticles as described in [18]) or by a combination of miniemulsion and emulsion/solvent evaporation techniques (PLLAnanoparticles without and with magnetite, as described in [19][20]). In all cases, SDS was used as a surfactant for the synthesis or formation of the nanoparticles
previously demonstrated [18]. In addition to the good uptake properties, the polystyrene particles did not have any cytotoxic effect (Figure 1B). The biodegradable PLLAnanoparticles also showed good uptake and no cytotoxic effects (Figure 1A,B). This was also true for nanoparticles with iron oxide
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Figure 1:
(A) Nanoparticle uptake after 24 h incubation of hMSCs with 300 µg/mL nanoparticles analyzed by flo...
Beilstein J. Nanotechnol.2014,5, 1905–1917, doi:10.3762/bjnano.5.201
biological environments, intracellular degradation processes have been examined only to a very limited extent. PLLAnanoparticles with an average diameter of approximately 120 nm were decorated with magnetite nanocrystals and introduced into mesenchymal stem cells (MSCs). The release of the magnetite
particles from the surface of the PLLAnanoparticles during the intracellular residence was monitored by transmission electron microscopy (TEM) over a period of 14 days. It was demonstrated by the release of the magnetite nanocrystals from the PLLA surface that the PLLAnanoparticles do in fact undergo
degradation within the cell. Furthermore, even after 14 days of residence, the PLLAnanoparticles were found in the MSCs. Additionally, the ultrastructural TEM examinations yield insight into the long term intercellular fate of these nanoparticles. From the statistical analysis of ultrastructural details (e.g
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Figure 1:
Flow cytometry measurements showing the relative fluorescence intensity of the cells for different ...